Q. Do I need to download an app now?
A: No-- we will send you a link for the webform once we know where you're going to sample.
Q. Do you have any guides to California wildlife?
You can access our iNaturalist project & guides here:
Project Page: http://www.inaturalist.org/projects/caledna
Amphibian & Reptile Guide: http://www.inaturalist.org/guides/4219
Bird Guide: https://www.inaturalist.org/guides/4217
Fish Guide: http://www.inaturalist.org/guides/4221
Mammal Guide: http://www.inaturalist.org/guides/4218
Q. How long will it take my eDNA kit to arrive?
Q. How should I store my eDNA kit after sampling?
Try to send the CALeDNA kit back to us as soon as possible. If you will do this within 1 day of sampling keep the CALeDNA kit somewhere safe, at room temperature and out of direct sunlight. If it will take you a couple of days to send the CALeDNA kit back to us keep it in the refrigerator.
Q. How quickly should I send back my eDNA kit?
As quickly as possible. DNA degrades over time and microbial communities can change quickly, especially in novel environments like the collection tube so sending your CALeDNA kit back as quickly as possible is vital. We have included a return mailing postage sticker or a PDF. If you didn't get one, email us! email@example.com
Q. What is the experimental design?
A. You get to choose! We ask that you choose sites where you collect from different habitats if possible. Three replicate tubes are used at a single site. Replicate tubes are labeled R1,R2,R3 and should be collected 1 foot (30cm) apart from each other.
11. What should I take on a sampling trip?
eDNA - DNA deposited by animals, plants and microbes in the environment as they exist/pass through it. For example, it can contain a mixture of skin cells, fur, feces, blood, pollen, and leaves in tiny fragments in soil or sediment. The DNA may still be in the fragments or it may be loose and just chemically bound to soil particles.
Location - One of 3 main areas visited to collect samples. Within each location you will choose 2 sites and take 3 replicate samples at each site.
Replicate - One of multiple samples taken at a single site. We take multiple samples at the same site because obtaining DNA from a single species in multiple replicates provides stronger evidence of presence than a single sample which may have been accidentally contaminated.
Sample - Soil or sediment collected in the field and sent back to CALeDNA for genetic analyses.
Sediment - Organic matter and sand, silt, or clay that settles below the water.
Site - Area within a location where samples are collected. There are two sites per location and 3 samples taken per site.
Soil - A mixture of organic matter, minerals, gases, liquid and organisms that supports life on earth. We want you to take samples of surface soil so no digging is required.
Field Project -
Research Project -
Sample processing -